Expression of TNF-α and TGF-β1 in decidua tissue of patients with embryo damage
Objective To investigate the relationship between the expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in decidual tissue and embryo damage.Methods From November 2020 to October 2021,40 patients attending out-patient clinic of obstetrics and gynecology of the hospital diagnosed with embryo damage by B-ultrasound were selected as the observation group,and 40 nor-mal pregnant women who required artificial abortion during the same period were selected as the control group.Immunohistochemistry and reverse transcription real-time quantitative PCR (RT-qPCR) were used to detect the expression levels of TNF-α and TGF-β1 and their mRNA in decidua tissues,and the expression lev-els were compared between the two groups.The patients were divided into different subgroups with the mean values of TNF-α and TGF-β1 mRNA expression in decidua tissues as critical values,and the relationship be-tween TNF-α and TGF-β1 mRNA expression levels and embryo damage was further analyzed.Results Immu-nohistochemistry results showed that the positive expression rates of TNF-α and TGF-β1 in decidua tissues were 80.0％ and 52.5％ in the observation group,and 47.5％ and 75.0％ in the control group,respectively. The expression of TNF-α in decidua tissues of the observation group was stronger than that of the control group,the expression of TGF-β1 in decidua tissues of the observation group was weaker than that of the con-trol group,and the differences were statistically significant (Z=-3.622,3.898,P<0.05).RT-qPCR results showed that the relative expression level of TNF-α mRNA in decidua tissues of the observation group was higher than that of the control group (6.16±1.25 vs. 1.02±0.02),while the relative expression level of TGF-β1 mRNA of the observation group was lower than that of the control group (0.59±0.19 vs. 1.03± 0.03),and the differences were statistically significant (t=26.144,-14.199,P<0.05).The relative expres-sion levels of TNF-α and TGF-β1 mRNA in decidua tissues of the two groups were analyzed with the critical values of 3.59 and 0.81,respectively,which showed that the relative expression levels of TNF-α and TGF-β1 mRNA were related to the occurrence of embryo damage (OR=0.050,95％CI:0.013-0.193,P<0.001;OR=0.125,95％CI:0.055-0.284,P<0.001).Conclusion The increased expression of TNF-α and the de-creased expression of TGF-β1 in decidua might be associated with embryo damage.
万方数据
维普
