Effects of MEBT/MEBO on expression of CaN/NFAT signaling pathway in wound surface of diabetic rats
Objective To investigate the effect of skin regeneration medical technology[moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)]on the expression of calcineurin(CaN)/nuclear factor of activated T cells(NFAT)signaling pathway in the wound surface of diabetic rats.Methods A total of 48 SPF Wistar male rats were divided into the control group,model group(diabetic rat wound surface mod-el),MEBO group(diabetic rat wound surface model+MEBT/MEBO treatment)and Beifuxin(rb-bFGF)group(diabetic rat wound model+rb-bFGF treatment)according to the random number table method.After the wound model of diabetic rats was successfully made,the rats were treated with medicine immediately.On 3,7,14 d,4 rats were randomly selected from each group to take pictures and record the wound healing.The wound healing rate was calculated,HE staining was performed,the detected was conducted by quantitative re-al-time PCR(qPCR)and immunofluorescence.The one-way analysis of variance and LSD test were used for statistical analysis.Results On 3 d of treatment,there was a statistically significant difference in the wound surface healing rate between the control group and model group(P＜0.05).On 7,14 d of treatment,com-pared with the model group,the wound surface healing rate of the control group,MEBO group and the rb-bF-GF group was higher(P＜0.05).On 3,7,14 d of treatment,the NFAT and CaN expression levels in the con-trol group,MEBO group and rb-bFGF group were significantly higher than those in the model group,and the NFAT mRNA expression level in the control group and MEBO group were significantly higher than that in the model group(P＜0.05).On 14 d of treatment,the NFAT mRNA expression level in the control group was significantly higher than that in the model group(P＜0.05).On 3,7,14 d,the IL-2 expression level in the model group was higher than that in the control group,MEBO group and rb-bFGF group.Conclusion MEBT/MEBO could promote the healing of wound surface in diabetic rats by up-regulating the expression of CaN/NFAT signaling pathway.
nuclear factor of activated T cellsskin regeneration medical technologywound surface healingdiabetic ratscalcineurininterleukin-2
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