首页|粪便差异菌群、代谢物联合弥散张量成像对早产儿脑白质损伤的诊断价值

粪便差异菌群、代谢物联合弥散张量成像对早产儿脑白质损伤的诊断价值

Diagnostic value of fecal differential flora and metabolites combined with diffusion tension imaging in premature infants with white matter injury

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的 探讨粪便16S rRNA基因测序、代谢组学技术联合弥散张量成像技术(DTI)对早产儿脑白质损伤(WMI)的诊断价值.方法 选取2022年4-12月该院收治的出生胎龄＜32周且体重＜1 500 g的71例早产儿为研究对象,根据MRI检查结果分为 WMI组(n=23)和非 WMI组(n=48).收集出生后24 h内及出生后14、28 d的粪便标本,进行16S rRNA基因测序和LC-MS/MS代谢组学分析,纠正胎龄37～40周时完善头颅MRI+DTI检查,采用受试者工作特征(ROC)曲线分析肠道菌群结构、差异代谢物及DTI联合对WMI的诊断效能.结果 基于属水平诊断WMI的曲线下面积(AUC)为0.88(95％CI:0.74～＜1.00),WMI1和非WMI1差异代谢物集诊断 WMI的AUC为0.98(95％CI:0.96～0.99).胼胝体压部的ADC值(r2=0.230 2,P=0.001)、枕叶白质的FA值(r2=0.291 0,P=0.001)与肠道菌群结构最相关.差异性菌群、代谢物联合FA值(侧脑室旁白质和胼胝体压部)诊断WMI的AUC为0.98(95％CI:0.93～＜1.00).结论 特征性肠道菌群、差异代谢物和DTI参数可在很大限度上区分WMI和非WMI.
Objective To explore the diagnostic value of fecal 16S rRNA gene sequencing,metabolomics technology combined with diffusion tension imaging(DTI)in premature infants with white matter injury(WMI).Methods A total of 71 premature infants with gestational age＜32 weeks and body weight＜1 500 g treated in this hospital from April to December 2022 were selected as the research subjects and divided into the WMI group(n=23)and non-WMI group(n=48)according to the MRI results.The fecal samples were col-lected within 24 h after birth,on 14,28 d after birth,and 16S rRNA gene sequencing and LC-MS/MS metabo-lomics analysis were performed.All subjects underwent brain MRI+DTI examination when corrected gesta-tional age was 37-40 weeks.The diagnostic efficiency of the combination of intestinal flora structure,differ-ential metabolites and DTI on WMI was studied by receiver operator characteristic curve(ROC).Results The area under the curve(AUC)for diagnosing WMI based on the generic level was 0.88(95％CI:0.74-＜1.00).AUC of WMI1 and non-WMI1 differential metabolite set for diagnosing WMI was 0.98(95％CI:0.96-0.99).The ADC value of the splenium of corpus callosum(r2=0.230 2,P=0.001)and FA value of occipital white matter(r2=0.291 0,P=0.001)were most related to intestinal flora structure.AUC of differential flo-ra and metabolites combined with FA values(lateral ventricle white matter and corpus callosum pressure)in the diagnosis of WMI was 0.98(95％CI:0.93-＜1.00).Conclusion Characteristic intestinal flora,differen-tial metabolites and DTI parameters could distinguish WMI from non-WMI to a great extent.

white matter injury of preterm infantintestinal flora16S RNA sequencingmetabolomics analysis of LC-MS/MSdiffusion tension imaging

刘玲、罗晗、李银珊、李承燕、敖当、林少珠

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广东医科大学附属医院儿科,广东湛江 524000

早产儿脑白质损伤 肠道菌群 16S rRNA基因测序 LC-MS/MS代谢组学分析 弥散张力成像

广东省湛江市科技计划项目广东医科大学附属医院临床研究项目广东医科大学附属医院临床研究项目

2021A05078LCYJ2020DL01LCYJ2019B009

2024

10.3969/j.issn.1671-8348.2024.07.007

重庆医学
重庆市卫生信息中心,重庆市医学会

重庆医学

CSTPCD
影响因子：1.797
ISSN：1671-8348
年,卷(期)：2024.53(7)
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