Expression of POF1B in chorionic villi of pregnant women with spontaneous abortion and its related action mechanism
Objective To analyze the expression level of POF1B in the chorionic villi of pregnant women with spontaneous abortion and its related action mechanism.Methods The clinical data of 60 pregnant women with spontaneous abortion admitted and treated in the Eighth Affiliated Hospital of Sun Yat-sen Uni-versity(Futian,Shenzhen)from January 2021 to January 2023 were collected and 60 pregnant women with in-duced abortion during the same period served as the normal control group.The serum and chorionic villus POF1B protein expression levels in the two groups were detected by Western blot.The levels of serum β-hu-man chorionic gonadotropin(β-HCG),progesterone and estrogen were detected by enzyme-linked immunosor-bent assay,and Pearson correlation was used to conduct the correlation analysis.Human chorionic trophoblast cells were cultured and divided into the control group and POF1B overexpression group,the control group was not treated,and the POF1B overexpression group was transfected with overexpresion POF1B plasmid.The bi-ological behaviors such as cellular proliferation and apoptosis were compared between the two groups,and the Western blot was used to detect the expression levels of cellular cysteine aspartate protease 3(Caspase3),Jun dimerization protein 2(JDP2)and secretory phosphoprotein 1(SPP1)in the two groups.Results The serum and chorionic villus POF1B protein expression levels of pregnant women in the spontaneous abortion group were significantly higher than those in the normal control group(P＜0.05),and the serum β-HCG,progester-one and estrogen levels were significantly lower than those in the normal control group(P＜0.05).The Pear-son correlation analysis showed that serum POF1B level was negatively correlated with β-HCG,progesterone and estrogen(r=-0.516,-0.481,-0.618,P＜0.05),and chorionic villus POF1B level was negatively cor-related with β-HCG,progesterone and estrogen(r=-0.438,-0.468,-0.521,P＜0.05).The proportion of apoptotic cells in human chorionic villous trophoblast cells and Caspase3 protein expression in the POF1B overexpression group were higher than those in the control group(P＜0.05).At 0 h,there was no significant difference in the relative number of the cells between the two groups(P＞0.05).At 12,24,48 h,the relative number of the cells in the POF1B overexpression group was significantly lower than that in the control group(P＜0.05).The expression levels of JDP2 protein and SPP1 protein in the POF1B overexpression group were significantly higher than those in the control group(P＜0.05).Conclusion The high expression of POF1B in serum and chorionic villi of pregnant women with spontaneous abortion is negatively correlated with pregnant women related hormones.
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