Expression and clinical value of lncRNA MEG3 and miR-21 in peripheral blood of patients with systemic lupus erythematosus
Objective To investigate the expression level of long chain non-coding RNA MEG3(ln-cRNA MEG3)and miR-21 in peripheral blood of patients with systemic lupus erythematosus(SLE)and their clinical significance.Methods A total of 50 patients with newly active SLE in the People's Hospital Affiliated to Hubei University of Medicine from 2020 to 2021 were selected as the SLE group,and another 50 healthy people were selected as the control group.Reverse transcription polymerase chain reaction(PCR)was used to detect the difference of expression of lncRNA MEG3 and miR-21 in peripheral blood between the two groups.Serum complement C3 and C4,immunoglobulin(Ig)G,IgA and IgM were detected by colorimetric method and transmission method,and anti-ds-DNA antibody was detected by indirect immunofluorescence method.The levels of interleukin(IL)-1,IL-6,IL-8,tumor necrosis factor-α(TNF-α)and high-sensitivity C-reactive protein(hs-CRP)were detected by ELISA.Pearson and Spearman correlation analysis were used to evaluate the correlation between lncRNA MEG3 and miR-21 and clinical indicators and inflammatory factors,and the diagnostic efficacy of lncRNA MEG3 and miR-21 in SLE was evaluated by the receiver operating characteris-tics(ROC)curve.Results Compared with the control group,the levels of complement C3,C4 and lncRNA MEG3 in the SLE group were lower,while the levels of IgG,IgA,IL-1,IL-6,IL-8,TNF-α,hs-CRP and miR-21 and the positive rate of anti-ds-DNA were higher(P＜0.05).The expression of lncRNA MEG3 in the SLE group was negatively correlated with IgG,IgA,miR-21,L-1,IL-6,IL-8,TNF-α and hs-CRP,and positively correlated with complement C3 and C4(P＜0.05).MiR-21 was positively correlated with IgG,IgA,IL-1,IL-6,IL-8,TNF-α and hs-CRP,and negatively correlated with C3 and C4(P＜0.05).The ROC curve showed that the area under the curve of lncRNA MEG3 combined with miR-21 was 0.958(95％CI:0.926-0.990),and the sensitivity,specificity and Youden index were 92.0％,86.0％and 0.780,respectively.Conclusion The ex-pression level of lncRNA MEG3 in SLE patients is decreased,and the expression level of miR-21 is increased.The combination of lncRNA MEG3 and miR-21 has certain diagnostic value for SLE.
systemic lupus erythematosuslong chain non-coding RNA MEG3miR-21diagnosisclini-cal value
万方数据
维普
