Study on application effect of fine needle aspiration cytology combined with multi-gene detection in diagnosis of thyroid nodules
Objective To investigate the expression characteristics of BRAF,RAS and TERT genes in ultrasound-guided fine needle aspiration specimens and their application value in the preoperative diagnosis of thyroid nodules.Methods A retrospective analysis was performed on 537 cases of fine needle aspiration speci-mens of thyroid nodules in the Aviation General Hospital.The cell smear and liquid-based cell section prepara-tion from each specimen conducted the Papanicolaou's stain and the results were interpreted according to the Bethesda grading system.The gene mutation situation in punctured tissues was analyzed by real-time quantita-tive PCR.Results There were 65 cases (12.10％) of Bethesda class Ⅰ nodules,153 cases (28.49％) of class Ⅱ,84 cases (15.64％) of class Ⅲ,9 cases (1.68％) of class Ⅳ,73 cases (13.59％) of class Ⅴ and 153 cases (28.49％) of class Ⅵ.There were 220 cases (40.97％) of BRAF V600E mutation,21 cases (3.91％) of NRAS mutation,6 cases (1.12％) of KRAS mutation and 2 cases (0.37％) of HRAS mutation.Among them,there was BRAF V600E and KRAS co-mutation in 1 case.BRAF V600E gene mutation had the correlation with cytological diagnostic results (P<0.05),BRAF V600E mutation was more likely to occur in younger ones (P<0.05),and BRAF V600E mutation was more common in males than in females (P<0.05).KRAS mutation was more likely to occur in nodules in the left lobe of the thyroid gland (P<0.05).Conclusion BRAF V600E gene mutation is the most common among thyroid fine needle puncture specimens,and the NRAS mu-tation is in the majority for RAS gene mutations.
万方数据
维普
