Construction of teaching experimental conditions for determination of drug antagonism parameters with bullfrog as experimental object
Objective To explore the application of bullfrog as the experimental object in pharmacoki-netic experiment drug dose-effect curve drawing and competitive antagonist pA2 determination.Methods The rectus abdominis muscle of bullfrog served as the sample.The semi-logarithmic molal concentration accumula-tion method was used.The effect of competitive antagonist-tubocurarine on the contraction action of acetyl-choline(ACh)agonist was observed.Results The rectus abdominis of bullfrog(regardless of gender)weighed 200-250 g served as the sample.The ACh solution of 1.3 × 10-4-1.3 × 10-1 mol/L series concen-trations could cause the contraction reaction of most specimens when adding the second and third doses,more-over which could make the contraction of the sample to reach the maximum effect;0.4X 10-3 mol/L tubocura-rine solution could effectively antagonize the effect of Ach;in addition,the contraction effect generated the specimen was highly correlated with the effect of bullfrog or toad as the experiment animal.Conclusion The determination conditions of bullfrog antagonistic parameters could meet the experimental requirements.
万方数据
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