Study on promoting healing performance of seaweed fiber dressing combined with epidermal growth factor
Objective To evaluate the effect of self-made seaweed fiber dressing combined with epider-mal growth factor(EGF)for promoting the wound healing.Methods The micro-morphology and structure,air permeability and chemical structure of the prepared seaweed fiber dressings were tested.The effects of dif-ferent concentrations of dressing leach liquor[0(control group),100％(dressing group)]on the cell activity and cellular survival of human embryonic lung fibroblasts(HELF)were detected by the cell counting kit-8(CCK8)and Calcein-AM/PI.The mouse model of full-layer back skin defect wound was constructed and di-vided into the model group,gauze group,gauze+EGF group,dressing group and dressing+EGF group ac-cording to different treatment methods,12 cases in each group.The wound healing of mice after skin injury in each group was observed and the wound healing rate on 3,7,11,15 d was calculated.The tissues of wound sur-face and surrounding skin were taken on 3,7,11 d of heal for conducting the tissue embedding section,hema-toxylin-eosin staining and Masson staining.The immunohistochemistry was used to observe the growth situa-tion of new epithelial,degree of inflammatory cell infiltration and deposition of collagen fibers in the wound surface.Results The inside of the seaweed fiber dressing showed the three-dimensional network structure and smooth surface.After drying treatment,there was no residual fiber attachment with good air permeability.The cell survival rate and cell viability had no statistical difference between the dressing group and control group(P＞0.05).On 3 d of heal,the healing rate of wound surface in the dressing+EGR group was the highest among the various groups(P＜0.05),on 15 d,except for the gauze group and model group,the heal in the other groups was good.The HE staining showed that on 7 d of healing,the length of the new epithelium in the dressing+EGF group was longer and the inflammatory cells were less than those in the other groups(P＜0.05).On 11 d,the number of inflammatory cells had no statistical difference among the dressing group,gauze+EGF group and dressing+EGF group(P＞0.05),but all were less than that in the gauze group and model group(P＜0.05).CD31 immunohistochemistry and Masson staining showed that the CD31 positive ex-pression on 11 d of heal in the dressing+EGF group was stronger,the new vessels were more,the vessel di-ameter was greater and collagenous fiber was densest.Conclusion Seaweed fiber dressing is porous,breath-able,hygroscopic and biocompatible,non-cytotoxic and absorbable.Combining with EGF has good promotive effect for the growth of new epithelium,could slow down the infiltration of inflammatory cells and promote the formation of new blood vessels and the deposition of collagen fibers
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