首页|Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

Unraveling the mechanism of action of Shangxia Liangji formula for treating insomnia:a metabolomics and network pharmacology approach

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Background:Insomnia is a prevalent clinical condition and Shangxia Liangji formula(SXLJF)is a well-established method of treatment.Nevertheless,the specific mechanism of action of SXLJF remains unclear.Methods:The mouse model of insomnia was established by intraperitoneal injection of para-chlorophenylalanine.Forty-two mice were randomly divided into a negative control group,model group,SXLJF group(18.72 g/kg/day),and positive control group(diazepam,2 mg/kg)and treated with the corresponding drugs for 7 consecutive days.The open field test and pentobarbital-induced sleeping test were conducted.LC-MS-based untargeted metabolomics and network pharmacology were applied to explore the potential targets of SXLJF for treating insomnia.Finally,key targets were validated using RT-qPCR.Results:Behavioral tests demonstrated that SXLJF reduced the total distance,average velocity,central distance,and sleep latency,and prolonged sleep duration.Metabolomics and network pharmacology revealed potential targets,signaling pathways,metabolic pathways,and metabolites associated with the anti-insomnia effects of SXLJF.Specifically,tyrosine hydroxylase(TH)and tyrosine metabolism emerged as crucial metabolic pathways and targets,respectively.RT-qPCR results supported the role of TH in the mechanism of SXLJF in treating insomnia.Conclusion:In conclusion,TH and tyrosine metabolism may represent significant targets and pathways for SXLJF in treating insomnia.

Shangxia Liangji formulainsomniametabolomicsnetwork pharmacologytyrosine hydroxylasetyrosine metabolism

Xia-Jie Quan、Hao Liang、Yong-Hong Tang、Li Jiang、Xiong-Ying Ji、Feng-Ying Zhang、Ping Zhang、Bo Ouyang

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Department of Information,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China

Department of Neurology,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China

Department of Gastroscopy,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China

Department of Traditional Chinese Medicine,The Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hengyang 421002,China

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2025

10.53388/TMR20240513002

传统医学研究(英文版)
天津市金匮灵兰科技有限公司

传统医学研究(英文版)

ISSN：2413-3973
年,卷(期)：2025.10(2)