The Feeding Habits of Four Silkworm Varieties(Bombyx mori) on an Artificial Diet and the Detection of Gene Expression Associated with Feeding Behavior
The use of artificial feed enables factorial sericulture and the study of silkworm genetic mecha-nisms of feeding behavior as well as the breeding of silkworm varieties for artificial feed,which is an essen-tial basis for achieving this goal.Whole-age artificial feed and whole-age mulberry leaf diets were applied separately in four silkworm varieties including A Ji,872,Qing Song,and Su Chun to investigate the weight of fifth instar silkworm and feeding habit of fifth instar silkworm.Results showed a significant difference between 872 and Su Chun,but there is no significant difference between A Ji and Qing Song by two feeding regimes.In whole-age artificial feed group,more than 80％ fifth instar larvae of the three vari-eties of A Ji,872 and Qing Song chose artificial feed,while only 20％ of Su Chun silkworms chose artifi-cial feed.Under the full-age mulberry leaf feeding,the fifth instar larvae of the three varieties of 872,Qing Song and Su Chun all chose mulberry leaf diet,only 70％ of A Ji chose mulberry leaf feeding.Further,the fifth instar larvae mandibles on day 3 of Su Chun with significant different body weight and the worst feed-ing ability of artificial diet and A Ji with no significant different body weight and good feeding ability of ar-tificial diet were selected as tissue samples,and the expression differences of general odorant binding pro-tein genes,GOBP1,GOBP2 and odorant receptor gene,OR56 of 2 different breeds were analyzed by real-time fluorescence quantitative PCR (qRT-PCR).The results showed that the expression of GOBP 1 and GOBP2 was higher in Su Chun than A Ji (P<0.01,P<0.001),but the expression of OR56 in A Ji was higher than Su Chun (P<0.05).It is found that the feeding habits of artificial feed of silkworm influenced by variety and the expression level of olfactory related genes,which is different among varieties with dif-ferent diets.This could serve as one of the foundational criteria for the selection and breeding of silkworm varieties adapted to artificial feed.
Artificial feed of silkwormWeight of 5th instarFeeding habit of 5th instarOlfactory related genes
万方数据
维普
