Identification and Preliminary Evolution Analysis of Insect Cocoonase
The relationship between the evolution of cocoonases in insects and their physiological adapta-bility is a research hotspot in biological evolution.However,the sequence characteristics and identification of cocoonase are still unclear.In this study,the MEME Suite analysis was used to obtain 4 signature mo-tifs of cocoonase.The distribution of cocoonase in 105 insects was analyzed by HMMER and BLAST pro-grams.The results showed that this gene was identified only in 36 species of Lepidoptera insects,and even significant gene duplication was found especially in the Nymphalidae (such as Pteropodidae).The activity and stability of cocoonase and trypsin in silkworm saliva were initially simulated by Gromacs.As a result, the final energy of cocoonase was lower,indicating that it was more suitable for the environment of silk-worm spit.These results laid the foundation for further analysis of the evolutionary and function of cocoonase.
万方数据
维普
