Sex determination is a fundamental and important scientific issue in biological research.There are differences in physiological,biochemical and economic characters between male and female silkworms.The sex determination of silkworms has always been an important subject in sericulture science and indus-try.In this paper,studies on sex chromosome,sex-determining genes,sex-determining cascade,and the application of sex determination in the industry of silkworm were summarized to provide reference infor-mation for further analysis of sex determination regulatory network of silkworm,elucidating sex determi-nation regulatory mechanism of silkworm,and promoting the study of sex determination in other lepidop-tera insects.