Identification and Expression Pattern Analysis of the Monocarboxylate Transporter Gene Family in Silkworm,Bombyx mori
Monocarboxylate transporter(MCT)is an important transmem brane transporter on the cellmem brane which is responsible for the transmem brane transport of mono-carboxylic acids such as lac-tic acid and pyruvate,and participates in the regulation of metabolic homeostasis.In this study,we identi-fied the MCT family members in silkworm genome by bioinformatics methods,and their expression pat-terns were detected by qRT-PCR.The results showed that there were 21 MCT family genes in the silk-worm genome,with the length of the encoded amino acid being 438～791 amino acids and the isoelectric point between 5.47～9.69.Analysis reveals that the MCT family genes of Bombyx mori are distributed on seven chromosomes,with tandem repeats present on chromosomes 5,7,13 and 23.All genes in this fami-ly encode proteins containing more than six conserved motifs,with MCT9 protein containing 12 trans-membrane domains,similar to homologous proteins in Homo sapiens,Drosophilidae,Apis mellifera,Tribolium castaneum and Xenopus laevs.The results of cluster analysis show that among the 21 mem-bers,15 are clustered in one branch with MCT protein members in insects such as Apis mellifera and Tribolium castaneum.In humans,only four MCT protein sequences are clustered with insects,and the re-maining 10 are clustered into a separate branch.Further detection of the expression patterns of these genes in different tissues of Bombyx mori shows that BmMCT5 is highly and specifically expressed in the mid-gut,while BmMCT1,BmMCT7,BmMCT9-2,BmMCT14-2,and BmMCT18 are highly and specifically expressed in the mapighian tubules.It is speculated that they participate in life activities such as digestion and excretion of Bombyx mori.The results of this study provide a theoretical basis and data support for in-depth exploration of the biological functions of the MCT family of silkworms.
万方数据
维普
