蚕学通讯2024，Vol.44Issue(3) ：85-88.

利用孤雌生殖纯合有荧光标记的家蚕育种材料的实验

Parthenogenetic Technology was Used to Perform Homozygous Experiments on Fluorescently Labeled Silkworm Breeding Materials

何行健 龚椿营 贾超华 陈卓华 王晓莉 乔匿骎 申林静 吴争雄 王俊 刘洋 艾均文
蚕学通讯2024，Vol.44Issue(3) ：85-88.
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  • 维普
  • 万方数据

利用孤雌生殖纯合有荧光标记的家蚕育种材料的实验

Parthenogenetic Technology was Used to Perform Homozygous Experiments on Fluorescently Labeled Silkworm Breeding Materials

何行健 1龚椿营 1贾超华 1陈卓华 1王晓莉 1乔匿骎 1申林静 1吴争雄 1王俊 1刘洋 1艾均文1
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作者信息

  • 1. 湖南省蚕桑科学研究所,长沙 410127
  • 折叠

摘要

在基因编辑家蚕育种材料选择和纯化阶段,采用温汤处理法对2个带有荧光标记的材料进行了孤雌生殖诱导实验,共获得138个有效卵圈.调查不同育种材料的孤雌生殖发生频率,结果显示2个材料的孤雌生殖发生频率在4.61％～97.06％之间,其中,材料HYB-U的孤雌生殖发生频率较高,平均为78.17％,而材料C9K-F的发生频率较低,平均为40.80％.通过饲养实验,成功获得了材料C9K-F的2个孤雌生殖存活个体,为后续的基因编辑家蚕育种材料培育和孤雌生殖研究打下了实验基础.

Abstract

In the selection and purification stage of gene-edited silkworm breeding materials,partheno-genesis induction experiments were carried out on two fluorescently labeled materials by warm soup treat-ment,and a total of 138 effective egg batches were obtained.The frequency of parthenogenesis in different breeding materials was investigated,and the frequency of parthenogenesis in the two materials was be-tween 4.61％～97.06％,among which the frequency of parthenogenesis in HYB-U was higher(78.17％on average),while the frequency of parthenogenesis in C9K-F was lower(40.80％on average).Through the breeding experiment,two parthenogenetic surviving individuals of C9K-F were successfully obtained,which laid an experimental foundation for the subsequent research on the cultivation of gene-edited silk-worm breeding materials and parthenogenesis.

关键词

孤雌生殖/温汤处理/发生频率/家蚕育种材料/荧光标记

Key words

Parthenogenesis/Warm soup treatment/Frequency of occurrence/Silkworm breeding ma-terials/Fluorescent labeling

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出版年

2024
蚕学通讯
重庆市蚕丝学会,西南大学

蚕学通讯

影响因子：0.139
ISSN：1006-0561
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