The Antimicrobial Mechanism of Tea Polyphenols Against Shewanella putrefaciens
Antimicrobial effect and mechanism of TP against Shewanella putrefaciens in vitro was investigated. The results showed that the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of TP against the S. putrefaciens were 1 mg·mL-1 and 16 mg·mL-1, respectively. Growth curves of S. putrefaciens were significantly inhibited in the addition of 2 mg·mL-1 TP. The population of S. putrefaciens decreased about 105 CFU·mL-1 and 106 CFU·mL-1 with the 2 mg·mL-1 TP addition after 96 h at 25℃ and 192 h at 4℃, respectively. The bactericidal activity of TP exhibited the time depending. Among the four monomer of TP, the order of bactericidal activity were EGCG>ECG>EGC>EC. The enzyme activity of Na+/K+-ATP and AKP in the culture suspension of S. putrefaciens gradually increased with the time extension in the TP treated samples. TEM images revealed that treatment of TP might lead to damage and deformation of cell. Thus, TP exhibited the good antimicrobial and bactericidal activity against S. putrefaciens by disrupting cell walls and out membrane, which cause the permeability of intracellular components and the change of cell morphology.
国家科技期刊平台
NSTL
万方数据
维普
