普洱茶素Ⅴ～Ⅶ对4种乳腺癌细胞作用研究
Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells
杨锐 1赵兴平 1何明婕 1刘敏 1罗蓉 1陈川龙 2潘淑康 1丁章贵3
作者信息
- 1. 云南大益微生物技术有限公司,云南 昆明 650217;云南省普洱茶发酵工程研究中心,云南 昆明 650217;农业农村部普洱茶加工技术重点实验室,云南 昆明 650217
- 2. 大益集团勐海茶业有限责任公司,云南 勐海 666200;农业农村部普洱茶加工技术重点实验室,云南 昆明 650217
- 3. 云南大益微生物技术有限公司,云南 昆明 650217;云南省普洱茶发酵工程研究中心,云南 昆明 650217;云南大学生命科学学院云南省微生物研究所,云南 昆明 650091;云南大学西南微生物多样性教育部重点实验室,云南 昆明 650091;农业农村部普洱茶加工技术重点实验室,云南 昆明 650217
- 折叠
摘要
普洱茶素(Puerins)是从普洱熟茶中分离得到的儿茶素衍生物,是一类结构独特的含氮多酚化合物.开展普洱茶素Ⅴ～Ⅶ抗乳腺癌细胞作用的研究,以 4 种不同受体表型的乳腺癌细胞株为模型,进行噻唑蓝(MTT)比色试验、蛋白质组学检测及细胞凋亡检测等试验.结果表明,普洱茶素Ⅴ、Ⅵ和Ⅶ等 3 个单体化合物对 4 种乳腺癌细胞都有显著的抑制作用;蛋白质组学分析发现,普洱茶素干预后乳腺癌细胞表达产生差异的蛋白主要集中于代谢通路,其次是癌症通路和黏附斑信号通路;细胞凋亡检测发现 3 个普洱茶素化合物均可诱导MDA-MB-231 细胞的凋亡.本研究报道了普洱茶素Ⅴ～Ⅶ的抗乳腺癌细胞活性及初步作用机制,为研究普洱熟茶抗癌活性物质提供基础信息.
Abstract
Puerins,a kind of nitrogen-containing polyphenol compound with unique structure,is a kind of catechin derivative first isolated from ripened Pu-erh tea.In this paper,the effects of puerins Ⅴ-Ⅶ on breast cancer cells were studied.Four breast cancer cell lines with different receptor phenotypes were used as models,and MTT assay,proteomics detection and apoptosis detection were carried out.The results show that all puerins Ⅴ,Ⅵ and Ⅶ had obvious inhibitory effects on the four kinds of breast cancer cells.Proteomics analysis indicates that the differentially expressed proteins were mainly concentrated in the metabolic pathway,followed by cancer pathway and adhesion spot signal pathway under the intervention of puerins Ⅴ-Ⅶ.Apoptosis detection demonstrates that all three puerins compounds could induce the apoptosis of MDA-MB-231 cells.This study first reported the anti-breast cancer activity and preliminary mechanism of puerins Ⅴ-Ⅶ,which provided basic information for studying the anti-cancer active substances of ripened Pu-erh tea.
关键词
普洱熟茶/普洱茶素/乳腺癌/蛋白质组学/细胞凋亡Key words
ripened Pu-erh tea/puerins/breast cancer/proteomics/apoptosis引用本文复制引用
基金项目
云南省重大科技专项(202002AE32001)
兴滇英才支持计划(YNWR-QNBJ-2019-003)
出版年
2024
国家科技期刊平台
NSTL
万方数据