Study on the Effects of Puerins Ⅴ-Ⅶ on Four Kinds of Breast Cancer Cells
Puerins,a kind of nitrogen-containing polyphenol compound with unique structure,is a kind of catechin derivative first isolated from ripened Pu-erh tea.In this paper,the effects of puerins Ⅴ-Ⅶ on breast cancer cells were studied.Four breast cancer cell lines with different receptor phenotypes were used as models,and MTT assay,proteomics detection and apoptosis detection were carried out.The results show that all puerins Ⅴ,Ⅵ and Ⅶ had obvious inhibitory effects on the four kinds of breast cancer cells.Proteomics analysis indicates that the differentially expressed proteins were mainly concentrated in the metabolic pathway,followed by cancer pathway and adhesion spot signal pathway under the intervention of puerins Ⅴ-Ⅶ.Apoptosis detection demonstrates that all three puerins compounds could induce the apoptosis of MDA-MB-231 cells.This study first reported the anti-breast cancer activity and preliminary mechanism of puerins Ⅴ-Ⅶ,which provided basic information for studying the anti-cancer active substances of ripened Pu-erh tea.
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