Study on callus induction and plant regeneration with explant of alfalfa
The hypocotyl, leaf ,cotyledon and root of Medicago sativa var. Zhongmu No. 1were used to study the effect of hormones in different concentrations on embryogenic callus induction and the effect of different medium and sucrose concentration on embryoid differentiation. The results showed that the induction from different explants varied. The callus induction rate from hypocotyl could reach 92. 2%;the best medium for embryogenic callus induction was the modified SH + 2,4-D 2. 0 mg/L+6-BA 0. 5 mg/L,it was MSO1 and MSO for embryoid differentiation and plant regeneration respectively. By adding 25 ~ 30 g/L sucrose,the highest embryoid differentiation could reach 30. 4%.
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万方数据
维普
