Effect of space induction on salt tolerance and plant regeneration of alfalfa
The impact of space induction on salt tolerance of alfalfa was studied by measuring germination rate and seedling regeneration ability with Zhongmu No. 1 alfalfa. Results showed that germination rate of induced seeds increased by 25. 7% ,the aberration rate of cotyledon and germination days decreased compared with that of the CK seeds under stress of 250 mmol NaCl. Space induction showed positive impact on the regeneration ability of alfalfa seedlings under salt stress. Compared with the control,the induced seeds showed higher ability in terms of formation and growth of callus, number of buds and roots. Based on the salt tolerance, 3 mutations were screened out.
alfalfamutationspace loadingsalt toleranceregeneration in vitro