Identification of plant growth promoting rhizobacteria Lycium ruthenicum and their effectives
In order to obtain plant growth-promoting rhizobacteria and clarify their growth-enhancing efficiency to Lycium ruthenicum,the root morphology and rhizosphere soil in L.ruthenicum Murr were collected.The strains of nitrogen-fixing and phosphate-solubilizing bacteria were separated with selective medium and the potential PGPR strains species were identified by physiological and biochemical characteristics and 16SrDNA sequence analysis.Result showed that the quantities of bacteria and PGPR represented a distribution trend of ‘rhizosplan or surface of roots (RP) ＞ soil adhering to roots (RS) ＞ histoplan or interior of roots (HP)’,that denoted a strong rhizosphere effect.We isolated 71 PGPR bacteria strains,which contained 21 nitrogen-fixing bacterial strains,30 inorganic phosphate-solubilizing bacterial strains and 20 organic phosphate-solubilizing bacterial strains.There were 7 strains of nitrogenase activity higher than 200 nmol C2H4/(h · mL),and 13 strains of phosphate solubilizing activity reached 300 μg/mL.A further 6 potential PGPR strains had been identified as Bacillus sp.This study offered potential PGPR for the development of microbial fertilizers and enriched the PGPR resources.
Lycium ruthenicumrplant growth promoting rhizobacteria (PGPR)characteristics of the growth
NETL
NSTL
万方数据
维普
