Optimization of the method for determining exopolysaccharides and culture conditions of Erwinia persicina Cp2
[Objective]To determine the content of exopolysaccharides(EPS)in seed-borne Erwinia persicina Cp2 present in alfalfa and identify its optimum culture conditions for achieving higher yield of EPS.[Method]A single-factor test was employed to optimize the determination method and culture conditions of Cp2 EPS.[Result]The optimized conditions for the phenol-sulfuric acid method were determined as follows:maximum absorption wavelength of 490 nm,color development time of 20 min,color development temperature of 100℃,phenol mass frac-tion of 5%,phenol dosage 0.80 mL,and concentrated sulfuric acid dosage of 5.00 mL.The optimized medium for Cp2 EPS production included soy peptone(20.00 g),dipotassium hydrogen phosphate(1.50 g),magnesium sulfate(1.50 g),starch(25.00 g),distilled water(1.00 L)maintained at pH 7.0～7.2,sterilization at 121℃for 15 min,and the enrichment culture temperature of 30℃.[Conclusion]The results revealed that the phenol-sulfuric acid method was superior to anthrone-sulfuric acid in determining the Cp2 EPS content,resulting in 365.91%increase in Cp2 EPS production compared to the previous method.This study provides a theoretical basis for the functional re-search and product development of Erwinia persicina strain Cp2 exopolysaccharides.
Erwinia persicinaexopolysaccharidesoptimization of culture conditions
万方数据
维普
