Effects of adding a growth-promoting agent and organic fertilizer to alpine meadow on soil enzyme activities
[Objective]To investigate the effects of biotrophic fungicides and organic fertilizers on the soil envi-ronment of alpine meadows,and to provide theoretical basis for the application of biotrophic fungicides in natural meadows and the management of meadow ecosystems in alpine areas.[Method]The alpine meadow in Maqu,Gan-nan served as the research object in a randomized block group study with four treatments:control(CK),biotrophic fungicide(PG),organic fertilizer(OF),and biotrophic fungicide and organic fertilizer with 1:1 dosage(PG + OF).Four treatments were established to examine the effects of biotrophic agents and organic fertilizers on soil physico-chemical properties,microbial carbon,nitrogen,and phosphorus(MBC,N,and P),as well as hydrolytic enzyme ac-tivities(AP,βG,CBH,and βX,NAG),and to explore the impacts on enzymes and enzyme stoichiometric ratios in al-pine meadows.[Result]Compared with the control(CK),treatment with PG,OF and PG+OF significantly re-duced conductivity,while increasing the content of MBC,N and P.The MBN content in the PG+OF treatment was significantly higher than in other treatments,indicating a significant interaction between biotrophic fungicides and or-ganic fertilizers that enhanced the soil microbial population.Additionally,compared with the control(CK),treatments PG,OF and PG+OF significantly increased the activity of hydrolases,along with higher levels of MBC,N,and P.Furthermore,compared with CK,treatments PG,OF and PG+OF significantly promoted the hydrolytic enzyme ac-tivity,where MBC,MBP,MBN and EC emerged as important driving factors for changes in enzyme activity and en-zyme stoichiometry ratio in soil.The enzyme stoichiometry ratio displayed significant variations among different treat-ments.[Conclusion]The application of biotrophic fungicides and organic fertilizers substantially increased the micro-organism count,enhanced microbial activity and positively influenced the alleviation of carbon and phosphorus sources deficiencies in the soil.The combined application of biotrophic fungicides and organic fertilizers demonstrated the most significant impact on soil improvement.
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