摘要
[目的]探究燕麦(Avena sativa)种带细菌及其促生功能多样性.[方法]采用传统培养方法分离纯化9个燕麦品种种带细菌,并定性分析其固氮、溶磷、产IAA能力和分解纤维素功能.[结果]共分离到34株细菌分离物,结合其形态特征及16S rDNA分 析,分离出的细菌隶属于原核生物界的4门19 属,其中优势门为厚壁菌门(Firmicutes),占比 56.00%;优势属为芽孢杆菌属(Bacillus),占比41.18%;34株燕麦种带细菌均具不同的促生功能,其中分属于芽孢杆菌属、埃希氏菌属(Escherichia)、假单胞菌属(Pseudomonas)、志贺氏菌属(Shigella)和大西洋杆菌属(Atlantibacter)的 9株细菌同时具备固氮、溶磷、产吲哚乙酸和分解纤维素功能.[结论]本试验共分离到34株细菌分离物,均具备不同的促生功能,其中 9株细菌兼具固氮、溶磷、产吲哚乙酸和分解纤维素功能,志贺氏菌属LFt4综合能力最强.
Abstract
[Objective]In order to explore the diversity of seed-borne bacteria of oat and their growth-promoting functions.[Method]9 seed-borne bacteria of oat cultivars were isolated and purified by traditional cul-ture methods,and their functions of nitrogen fixation,phosphorus solubilization,IAA production and cellulose decom-position were qualitatively analyzed.[Result]A total of 34 bacterial isolates were isolated.Combined with their mor-phological characteristics and 16S rDNA analysis,the isolated bacteria belonged to 4 phyla and 19 genera of prokary-otes,in which Firmicutes was the dominant phyla for 56.00%.The dominant genus was Bacillus sp.,accounting for 41.18%.34 strains of oat seed bacteria all had different growth-promoting functions.It belonged to the Bacillus sp.,Escherichia sp.,Pseudomonas sp.,Shigella sp.,and Atlantibacter sp.9 strains had the functions of nitrogen fixation,phosphorus solubilization,indole-acetic acid production and cellulose decomposition.[Conclusion]A total of bacte-rial 34 isolates were isolated in this experiment,all of which had different growth-promoting functions.Among them,9 strains had the functions of nitrogen fixation,phosphorus solubilization,indoleacetic acid production and cellu-lose decomposition.Shigella sp.LFt4 had the strongest comprehensive ability.
基金项目
国家自然科学基金(32060369)
国家自然科学基金(31460639)
兰州市人才创新创业项目(2019-RC-116)
甘肃农业大学青年导师基金(GAU-QDFC-2019-08)
甘肃省杰出青年基金(20JR10RA562)
NETL
NSTL
万方数据