Identification and phylogenetic analysis of the em-bryogenic marker gene BBM in kentucky bluegrass(Poa pratensis)
[Objective]Using bioinformatics methods,this paper aims to identify the embryogenic marker gene PpBBM of kentucky bluegrass and predict its parthenogenetic function through conservative motif alignment and evo-lutionary tree analysis.[Method]Based on the information from the Transcriptome database of kentucky bluegrass,the PpBBM gene was searched.The physical and chemical properties of PpBBMs protein were analyzed,and the phy-logenetic relationship and conservative motif differences of PpBBMs proteins were compared through bioinformatics software.[Result]A total of three PpBBMs genes with complete open reading frame were obtained,and the proteins encoded by them all contained two AP2 domains,among which PpBBM1 and PpBBM2 have the same motif type and number.The three PpBBMs proteins are acidic proteins located in the nucleus without transmembrane structure and signal peptide.Phylogenetic analysis showed that the menbers of BBM/BBML prdens of 30 species were polym-erized into five groups.Among them,PpBBM1 and PpBBM2 are clustered in the second branch and have the closest genetic relationships with BdBBM and SbBBML,respectively;PpBBML is located in the fifth branch and has the closest genetic relationship with ZmBBML.PpBBM1,PpBBM2,and PpBBML all have the bbm-1 motif unique to the BBM gene and the euANT2-4 motif specific to the AP2 subfamily.However,all motifs of the PpBBML protein exhibited variations in individual amino acid.[Conclusion]PpBBM1 and PpBBM2 genes are more likely to have the ability to induce parthenogenesis compared with PpBBML.
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