Effects of EDTA on seed germination of creeping bentgrass under heavy metal stress
[Objective]In order to study the effect of exogenous EDTA on seed germination of creeping bent-grass(Agrostis stolonifera L.)under heavy metal stress.[Method]The creeping bentgrass'Pennway'variety was used as the test material,and the creeping bentgrass under different concentrations of Cu,Cd and Pb stress was treated with EDTA,and the seed germination rate,germination potential,germination index,seedling leaf and root re-lated indexes of creeping bentgrass were determined.[Result]The results showed that creeping bentgrass had the strongest tolerance to Pb,followed by Cu and Cd.The application of 3 mmol/L EDTA had better germination index,leaf index and root index of creeping bentgrass under moderate and severe heavy metal stress.Among them,the ger-mination rate of creeping bentgrass seeds treated with 3 mmol/L EDTA under moderate Cu,mild Cd and moderate Pb stress increased by 16.67%,3.36%and 8.72%,respectively,compared with the controls.The leaf length in-creased by 30.99%,30.60%and 1.63%,respectively,and the root length increased by 35.93%,0.37%and 29.80%,respectively.[Conclusion]Through the comprehensive ranking of each treatment by the membership func-tion method,it was found that the application of 3 mmol/L EDTA had the best mitigation effect on heavy metal stress,and the mitigation effect on the three heavy metals was ranked as Pb>Cu>Cd.
EDTAheavy metal stresscreeping bentgrassseed germination
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万方数据
