Seasonal response of endophytic fungal diversity in roots of Potentilla parvifolia in alpine scrub meadow of eastern Qilian M ountains
[Objective]To determine the diversity and seasonal variation of culturable endophytic fungi in the roots of Potentilla parvifolia in alpine region.[Method]Potentilla parvifolia,a dominant shrub in the eastern Qilian Mountains was used as the test material.The endophytic fungi in root were isolated by tissue culture,and their sea-sonal diversity was analyzed by morphological identification combined with ITS molecular sequencing.[Result]71 pure fungi were isolated from the tissue of the golden dew rhizome in spring,summer and autumn,belonging to 8 taxa of 5 classes,5 orders,5 families,6 genera.There were some differences in the species and quantity of endophytic fungi in the roots of Potentilla parvifolia in different seasons.From the diversity index,Shannon-Wiener index and Simpson's diversity index showed as spring(1.57,0.75)>summer(1.26,0.69)>autumn(1.08,0.62).The di-versity of endophytic fungi in the roots in spring was higher than that in summer and autumn,but the dominant genera were Neurospora and Aspergillus.The endemic genera in spring were Penicillium and Torula;Ochroconis was en-demic in summer,and Botrytis was endemic in autumn.[Conclusion]The endophytic fungi were isolated from the roots of Potentilla parvifolia in different seasons,and the endophytic fungi colonization in the roots of Potentilla par-vifolia had seasonal differences,The diversity of endophytic fungi in the roots in spring was higher.
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