首页|蒙古黄芪内生细菌产IAA菌株筛选与培养条件优化

蒙古黄芪内生细菌产IAA菌株筛选与培养条件优化

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[目的]探寻蒙古黄芪内生菌的促生功能,挖掘优异微生物资源.[方法]采用前期从蒙古黄芪 品 系 甘 芪 1 号 根 中 分 离 纯 化 的 20 株 内 生 细 菌 菌 株(B1～B20,GenBank 序 列 收 录 号OM938262～OM938281)为研究对象,以纯培养液为对照(CK),利用Salkowski显色法对产吲哚乙酸(IAA)功能菌株鉴定,然后对优异菌株的培养条件进一步进行优化研究.[结果]甘芪1号根可培养内生细菌菌株,有12株菌株较CK有明显Salkowski显色反应,测量各菌株产IAA浓度依次为B16>B18>B10>B20>B8>B13>B11>B3>B4>B6>B1>B17,产IAA浓度范围为2.74～26.48 mg/L,平均10.10 mg/L,其中B16高稳产IAA(GenBank OM938262),平均产IAA浓度高达 26.48 mg/L,极显著高于其他菌株产IAA的水平.培养条件优化显示,B16 菌株培养最适温度为 15～25℃,在浓度为0.1%～7%的NaCl培养基均能正常生长,最适NaCl浓度为1%,最适pH为7.0,最适碳、氮源分别为酵母提取物和蛋白胨.培养条件优化后B16 增殖及IAA产能较优化前分别极显著提高 28.6%和53.5%.[结论]蒙古黄芪根内生细菌中产IAA菌株比例较高,是优异豆科药用植物微生物资源宝库,且筛选到一株高稳产IAA 菌株B16,经过培养条件优化可达到40.658 mg/L,是有较大的生态调控利用潜力的菌株.
Identification and selection of IAA-producing strains from endophytic bacteria of Astragalus membranaceus var.mongholicus root and optimization of culture conditions
[Objective]To explore the growth promoting capabilities of its endophytic bacteria and harness excel-lent microbial resources.[Method]We isolated and purified 20 endophytic bacterial strains(B1～B20,GenBank se-rial numbers OM938262～OM938281)from the roots of"Ganqi No.1".Using a pure culture medium as control(CK),we identified functional strains capable of producing indoleacetic acid(IAA)through salkowski chromogenic method.Subsequently,we optimised the culture conditions for the outstanding strains.[Result]The results showed that 12 strains of endophytic bacteria from the root of"Ganqi No.1"exhibited significant Salkowski color reactions compared to CK.Among them,strain B16 demonstrated the highest IAA production concentration,followed by B18>B10>B20>B8>B13>B11>B3>B4>B6>B1>B17,respectively.The IAA production con-centrations ranged from 2.74 to 26.48 mg/L,average 10.10 mg/L.Notably,strain B16(GenBank OM 938262)consistently produced IAA at a high concentration of 26.48 mg/L,significantly outperforming other strains.Optimiza-tion of culture conditions showed that strain B16 thrived in 0.1%～7%NaCl medium at temperatures ranging 15℃to 25℃,with the optimum NaCl concentration being 1%and the optimal pH level at 7.0.Yeast extract and peptone were identified as the optimal carbon and nitrogen sources respectively.Following optimization process,strain B16 ex-hibited a 28.6%increase in proliferation and a 53.5%enhancement in IAA production.[Conclusion]These findings highlight the prevalence of IAA-producing strain among the endophytic bacteria of Astragalus mongholicus root,un-derscoring their importance as a valuable resource for medicinal leguminous plants.Strain B16,in particular,emerged as a highly stable producer of IAA with its production reaching 40.658 mg/L post-culture condition optimization.This strain holds significant potential for ecological regulation and utilization.

Astragalus membranaceus var.mongholicusendogenous bacteriaindole-3-acetic acidstrain screen-ingculture conditions optimization

刘垒、郭凤霞、陈垣、周洋、李红玲、李琴、李国藩、王辉

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甘肃农业大学生命科学技术学院,农学院/甘肃省中药材规范化生产技术创新重点实验室,甘肃省药用植物栽培育种工程研究中心,甘肃省干旱生境作物学重点实验室,甘肃 兰州 730070

天津市现代中药资源研究企业重点实验室,天津天士力现代中药资源有限公司,天津 300410

蒙古黄芪 内生细菌 吲哚乙酸 菌株筛选 培养条件优化

省级现代丝路寒旱中药材产业发展项目(2021)甘肃省特色优势农产品评价陇西黄芪项目甘肃省科技支撑计划甘肃省中药材产业技术体系建设项目首席专家项目企业委托项目企业委托项目甘肃农业大学SRTP项目

2130122TYNPZ2020-181204FKCA123GARS-ZYC-1XDZY-2017-20181152019620005000036-2022620005001507202111018

2024

10.13817/j.cnki.cyycp.2024.02.015

草原与草坪
中国草学会　甘肃农业大学

草原与草坪

CSTPCD
影响因子：0.686
ISSN：1009-5500
年,卷(期)：2024.44(2)
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