Quality evaluation of free amino acids in branches and leaves of tamarixs based on multivariate statistical methods
[Objective]The aim of this paper is to explore and evaluate the quality of free amino acids in branches and leaves of tamarixs,and to screen out the excellent germplasm resources.[Method]Free amino acid composition in 12 tender branches and leaves of tamarix was measured using high performance liquid chromatography.Systematic evaluations were performed through correlation analysis,principal component analysis and the membership function method.[Result]The study revealed that the 12 tamarisks contained more than 17 types of free amino acids,includ-ing 7 essential amino acids,with total amino acid concentrations ranging from 863.21～1769.06 mg/kg and average content 1266.79 mg/kg.Various categories of amino acids were identified,including medicinal amino acid,sweet amino acid,bitter amino acid,flavor amino acid,aromatic amino acid and branched amino acid were 143.42～361.61 mg/kg,315.34～634.78 mg/kg,481.08～1279.89 mg/kg,117.41～341.62 mg/kg,159.90～374.71 mg/kg,22.83～118.58 mg/kg,49.40～107.90 mg/kg,and whose average were 226.80 mg/kg,472.81 mg/kg,715.39 mg/kg,181.38 mg/kg,242.06 mg/kg,48.93 mg/kg and 68.51 mg/kg,respectively.Six principal components were constructed by factor loading.The corresponding contribution ratio was 90.38%.Conelation analysis shoued that 33 fairs of amino acid indicaters shoued extremely sibnificant correlation(P<0.01),and 17 pairs of indicators shoued significant conelation(P<0.05).Listed by the amino acid quality,the top 4 members were Tamarix elongate Ledeb.,Tamarix gansuensis X.Z.Zhang,Tamarix ramosissima Ledeb.and Tamarix austromogoltca Nakai(Gansu).The last 4 members were Tamarix arceuthoides Bunge(NO.2),Tamarix chinensis Lour.,Tamarix ho-henackeri Bunge and Tamarix leptostachys Bunge.[Conclusion]Principal component analysis and the membership function method proved effective for evaluating the comprehensive quality of free amino acids in different tamarix spe-cies.Tamarix elongate Ledeb.,Tamarix gansuensis X.Z.Zhang,Tamarix ramosissima Ledeb.and Tamarix austro-mogoltca Nakai(Gansu)are recommended as excellent germplasm resources.This study lays a theoretical foundation for further breeding,development and utilization of tamarix species.
tamarisksfree amino acidprincipal componentmembership function method
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