Evaluation of germination characteristics and drought resistance of Artemisia wellbyi germplasm under PEG osmotic stress
[Objective]The aim of this research was toto evaluate the drought resistance of Artemisia wellbyi wild germplasm materials at the germination stage.[Method]We selected thirteen A.wellbyi germplasm materials collected from various habitats in Tibet as the research subjects and applied five osmotic stress treatments using a PEG-6000 concentration gradient of 0,-0.2,-0.4,-0.6 and-0.86 MPa.Our aim was to investigate the effects of PEG-6000 osmotic stress on the germination characteristics of A.artemisia,and comprehensively evaluate its drought resistance using Principal Component Analysis and Membership Function method.[Result]PEG osmotic stress reduced the relative germination potential,relative germination percentage,relative vigor index and germination stress index of A.wellbyi seeds.A.wellbyi responded to drought stress by delaying germination time,inhibiting bud length and promoting root length.Through principal component analysis,we identified five drought resistance in-dexes:relative germination potential,relative germination rate,relative root length,relative vigor index and germina-tion stress index.[Conclusion]Based on the comprehensive evaluation using two methods,materials No.5、11 and 4 ranked as the top three in drought resistance,while material No.13 ranked the lowest in drought resistance.
Artemisia wellbyiPEG osmotic stressprincipal component analysismembership function
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