Comprehensive evaluation of drought resistance of 7 Phleum pratense forage germplasm materials during seed germination
[Objective]In order to explore the effects of drought stress on seed germination and seedling growth of Phleum pratense,the drought-resistant germplasm was screened.[Method]In this study,6 wild Phleum pratense L.forage materials and 1 introduced species material were used as research objects.PEG was used to simulate drought stress,and 5 treatment gradients of 0%,5%,10%,15%and 20%were set.The germination indexes and growth indexes were determined,the differences of drought resistance indexes were analyzed,and the appropriate drought resistance identification concentration was determined and drought resistance germplasm was screened.[Result]The results showed that the low concentration of PEG(5%)could promote the root length,shoot length,seedling fresh weight and vigour index,while the high concentration of PEG(≥15%)would inhibit the germination rate,germination potential,germination index,root length,shoot length,root-shoot ratio and vigour index.And the root length of Phleum pratense L.was more sensitive than shoot length for drought stress.15%PEG was suitable for identifying drought resistance.Wild materials had better drought resistance potential.The results of principal compo-nent analysis and membership function comprehensive evaluation showed that the drought resistance of the seven ma-terials was MS>TM-3>TM-5>TM-6>TM-1>TM-2>TM-4 from strong to weak.[Conclusion]The research revealed that a certain degree of drought stress could promote seed germination and seed-ling growth of Phleum pratense,and could provide some reference for the screening of drought-tolerant germplasm and the breeding of new drought-tolerant varieties of Phleum pratense.
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