[Objective]This research was conducted to screen quinoa germplasm of different utilization values in arid and semi-arid regions of northwest China and to lay the foundation for the conservation and utilization of quinoa germplasm resources.[Method]Thirty-one quinoa germplasm resources collected from Dongxiang County,Linxia,Gansu Province were analyzed for their agronomic traits. Molecular marker PCR amplification was performed using ISSR primers to elucidate their genetic diversity and characteristics.[Result]The coefficients of variation for the five agronomic traits,such as plant height,stem thickness,fresh to dry ratio,stem to leaf ratio and yield per plant,ranged from 14.41% to 63.30%. Plant height showed a highly significant positive correlation with stem thickness,fresh to dry ratio and with yield per plant. Stem thickness was significantly positively correlated with yield per plant and fresh to dry ratio. Fresh to dry ratio was highly significantly correlated with yield per plant. Eight ISSR primers with high and clear polymorphism were selected and 63 bands were amplified,including 55 polymorphic bands. The ratio of polymorphic loci was 83.70%,the number of effective alleles (Ne) was 1.4515,the genetic diversity (H) was 0.2731 and the Shannon information index (Ⅰ) was 0.4174.With a genetic similarity coefficient of 0.72,the 31 qui-noa germplasm could be classified into five clusters.The first group was the selected longli 1 and longli 7,which have good resistance to lodging,and pests and diseases. The second group had low plant height which can be further se-lected as lodging resistant material. The third group had high plant height,high yield per plant,moderate fresh to dry ratio and small stem to leaf ratio.This group can be further selected as fresh fodder material.The fourth group had the largest fresh to dry ratio and was related to Taiwan Red Quinoa LQ-223. The fifth group had the largest fresh to dry ratio and was closely related to Taiwan Red Quinoa LQ-223.The fifth group was characterised by the largest stem to leaf ratio and medium maturity varieties.[Conclusion]The 31 quinoa germplasm resources in this experiment showed high genetic diversity,which can provide high-quality materials and scientific basis for quinoa germplasm re-source innovation and genetic breeding.