Screening of pseudomonas inorganic phosphate and its effect on ryegrass growth
[Objective]Study the ability of Pseudomonas to dissolve inorganic phosphorus and obtain strain re-sources with excellent characters.[Method]Eight strains of bacteria isolated from plant rhizosphere were inoculated into PKO medium and quantitative analysis was conducted using molybdenum antimony scandium method and mo-lecular biology identification. Semi-solid plant inoculation test was conducted to explore bacterial strain ability to dis-solve inorganic phosphorus and promote the growth of ryegrass. Preliminary identification was carried out.[Result]The dissolved inorganic phosphorus contents of eight bacterial strains were between 329.39 and 479.09 μg/mL after culturing in PKO liquid medium for 10 d,of the eight strains,strain MPD4 had the highest amount of dissolved phos-phorus. All eight bacterial strains belonged to the genus Pseudomonas.[Conclusion]The plant inoculation test showed that compared with the blank control,the eight strains could effectively promote the growth and development of ryegrass.This study provides an excellent strain resource for the further exploration of the growth-promoting effect of Pseudomonas phosphate-solubilizing bacteria and the production of microbial fertilizers.
万方数据
维普
