Effects of EMS mutagenesis on seed germination and seedling phenotypic characteristics of Astragalus cicer
[Objective]The study was carried out to explore the effect of EMS (Ethyl methyl sulfone) on the germination characteristics and phenotypic changes of Astragalus cicer seeds.[Method]Taking the seeds of A. cicer cv. Ganlv No. 2 as the test material,different concentrations (0.0％,0.2％,0.4％,0.6％,0.8％) of EMS were used for the treatment of the seeds for different time (2,4,6,8 h).[Result]The results showed that low concentra-tion and short-time EMS treatment promoted the germination of A. cicer seeds. With the increase of EMS concen-tration and the increase of mutagenesis time,the germination of A. cicer seeds was inhibited. The conductivity of the seed extract showed an upward trend with the deepening of the mutagenic conditions. At 2～6 h,the germination rate,germination potential,seedling length and root length at the germination stage first increased with the increase of treatment time and mutagenesis concentration,and then the germination rate and germination potential of seeds were the lowest when the EMS concentration was 0.8％,which were 54.45％ and 56.67％ lower than those of con-trol,respectively. Through scientific screening,it was found that the seedlings of A. cicer showed special variation characteristics such as dwarf seedlings,stagnant growth,yellow leaves,wide leaves,narrow leaves,purple leaves,and many disease spots.[Conclusion]Comprehensive analysis showed that 0.8％ EMS treatment for 6 h could be used as the best mutagenesis combination.The growth of seedlings was obviously inhibited with the increase of muta-genesis time and concentration,and the types of phenotypic variation also gradually increased.
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