Effects of tannic acid on the enzymatic activity of red and green pea aphids (Acyrthosiphon pisum)
[Objective]In order to determine the effects of tannic acid on the enzymatic activity of red and green Acyrthosiphon pisum.[Method]In this study,different concentrations of tannic acid (1 mg/L,3 mg/L and 5 mg/L) were fed with two color types of 5 d-aged pea aphid. SOD,POD,CarE and P450s enzyme activities in pea aphid were measured following different treatment times.[Result]The four enzymes responded differently to the different concentrations of tannic acid. With 24 h treatment,the maximum enzyme activities of CarE enzyme and P450 were 0.029±0.002 μmol/(μg·min) and 0.006±0.0009 μmol/(μg·min),respectively,which were significantly different (P＜0.05),when tannic acid concentration was 3 mg/L. The enzyme activities of SOD and POD of green A. pisum were the highest and reached 0.028±0.004 μg/mg and 1.679±0.172 μg/(mg·min),respectively,when tannic acid concentration was 3 mg/L at 72 h. These were significantly different from tannic acid concentration of 5 mg/L treat-ment (P＜0.05).[Conclusion]The different concentrations of tannic acid affected the activities of SOD,POD,CarE and P450 in red and green A. pisum . With the same treatment time,the activity of the same enzyme increased or de-creased to different degrees in the red and green type A. pisum.The activities of different enzymes in different concen-trations of tannic acid were different in the same type of A. pisum.
万方数据
维普
