Secondary metabolites and related genes in Didymodon vinealis under drought recovery stress
[Objective]Didymodon vinealis has a strong survival ability in desert. Study of the changes of me-tabolites,metabolic pathways and genes under hydration and drought stress provides theoretical basis for further study on drought tolerance of desert mosses.[Method]Rehydratration and drought treatments were performed in the soil with the same growth status,and the metabolome and transcriptome were determined in the samples over a one to eight day period.[Result]1) Principal component analysis (PCA) and orthogonal partial least square analysis showed significant differences in the metabolites of D. vinealis. The difference of metabolites over 4 days of rehydra-tion treatment was the largest,and the difference value was 55.The difference value of metabolites in 6-day drought samples was 49.178. The up-regulated metabolites under rehydration treatment were significantly higher than those under drought stress. 2) The high metabolites under rehydration treatment were:4-hydroxyphenylacetic acid,allan-toin,methyl-β-D-galactopyranoside,3-cyanoalanine,itaconic acid,2-aminophenol,lactic acid,Kyoto phenol,etc.The main metabolic pathways involved were fatty acid metabolism,purine metabolism,terpenoid metabolism,non-essen-tial amino acid metabolism,purine metabolism,phenolic metabolism and cell degradation metabolism.Its correspond-ing functional genes are:ga1A,rafA,maLZ,hisB,E2.7.1.138,CERK,E2.4.1.46,GPD1,ACTF,GALM,ahcy,CALM,EEFIB,phnX,CPK,desA,crtZ,etc.(3) After drought stress treatment,the metabolites with higher expres-sion were 2-monopalmitin,allantoin,arbutin,cholesteryl-3,5,6-triol,allylmalonic acid,pantothenic acid,etc. The main metabolic pathways involved are immune kinase metabolism,pigment metabolism,cholesterol metabolism,car-bon metabolism,amino acid biosynthesis,ABC transporter and purine metabolism. Functional genes include FAH,crtZ,SUR2,SCSDL,CYC,COX4,QCR7,UQCRB,galU,galF,UGP2,thiN,TPK1,SMARCC and so on.[Conclusion]Both rehydration and drought stress treatments resulted in the differential expression of key enzyme genes in the pathways of lactose metabolism,terpenoid biosynthesis and phenolic biosynthesis.The key enzyme genes were ga1A,hisB,CERK,GPD1,ACTF,CPK. The analysis of metabolites under different rehydration and drought stress treatments showed that the soil was not tolerant to flooding,but had strong tolerance to drought stress.
Didymodon vinealismetabolomestess of hydropenia and rehydtationdifferential metabolite and gene
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