Biological characteristics of ten herbage species and the effects of ultra-low temperature treatment on germination
[Objective]This study investigated the biological characteristics of seeds from ten herbage species and examined the effects of cryopreservation(liquid nitrogen) treatment on seed germination.The goal was to analyze how cryopreservation treatment influences germination and to explore changes in the thermodynamic properties of herbage seeds before and after treatment,providing a technical reference for the cryopreservation of herbage germ-plasm resources.[Method]Seeds from ten herbage species were used to determine their biological characteristics,in-cluding thousand-seed weight,seed length,seed width,width-to-length ratio,surface area,and plumpness rate. The moisture content of the seeds was reduced to 5％ using a weight reduction method.Germination rates before and after cryopreservation treatment were measured. Differential scanning calorimetry was employed to analyze the their-modynamic characteristics of the seeds.[Result]1) The seed morphology of the ten herbage species varied and in-cluded Avena sativa、Lolium multiforum、Festuca atundinacea、Leymus chinensis、Panicum intortum、Allium mongo-licum、Dendrolobium triangulare、Medicago sativa、Astragalus adsurgens、Trifolium repens. 2) A positive correla-tion was observed between seed plumpness and germination rate.3) Germination rate of the ten herbage seeds did not decrease after cryopreservation in liquid nitrogen;however,germination ability decreased and germination time was longer. 4) Differential scanning calorimetry analysis revealed that the moisture content and water state of the herbage seeds did not change after cryopreservation treatment.[Conclusion]The biological characteristics of seeds from ten herbage species varied. Seed plumpness rate can be used as an important indicator of seed germination ability. For cryopreservation of herbage germplasm resources,selecting seeds with high plumpness rate is crucial.When the mois-ture content of seeds is reduced to 5％,germination rate remain stable.Differential scanning calorimetry indicates that the water state of seeds does not change during cryopreservation treatment,making cryopreservation feasible for herb-age seeds.
herbage seedcharacteristics of morphologygerminationcryopreservation treatmentthermodynamic analysis
万方数据
维普
