Pseudomonas simiae MHR6产胞外多糖条件优化及抗氧化活性

Optimization of culture conditions and antioxidant activity of exopolysaccharides produced by Pseudomonas simiae MHR6

刘晓婷 ¹张琛 ¹韩江茹 ¹李晓林 ²姚拓¹

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作者信息

1. 甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州 730070
2. 金昌市金川区农业技术推广服务中心,甘肃金昌 737100
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摘要

[目的]研究培养条件对Pseudomonas simiae MHR6生长及胞外多糖(EPS)产量的影响,并探究其抗氧化活性,为新型农业生物制剂的开发利用提供可靠的理论依据.[方法]以EPS产量为指标,通过单因素试验对菌株MHR6产EPS条件进行优化,并对EPS的羟基自由基(OH-)、超氧阴离子(O2·-)和过氧化氢(H2O2)的清除作用进行了研究.[结果]经优化后,MHR6产EPS的最佳培养条件是:培养时间5 d、温度28℃、初始pH值8、接种量3％.当EPS质量浓度为1.0 mg/mL时,其OH-、O2·-和H2O2的清除率分别为56.94％、64.08％和31.69％.[结论]最佳培养条件下EPS产量达到最大值,为6.16 mg/mL,比优化前提高了24.9％.抗氧化实验结果表明MHR6所产的EPS具有良好的抗氧化活性.

Abstract

[Objective]In this paper,the effects of culture conditions on the growth of Pseudomonas simiae MHR6 and the production of exopolysaccharides(EPS) were studied,and the antioxidant activity of P. simiae MHR6 was investigated,providing a reliable theoretical basis for the development and utilization of new agricultural biological agents.[Method]With the yield of EPS as the index,the EPS production conditions by MHR6 was opti-mized by the single factor test. Subsequently,the antioxidant activity was determined by OH-,O2·-and H2O2 scav-enging rate of EPS.[Result]The results showed that after optimization,the optimal culture conditions for MHR6 to produce EPS were as follows:culture time 5 d,temperature 28 ℃,initial pH value 8,inoculation amount 3％. When the mass concentration of EPS was 1.0 mg/mL,the scavenging rates of OH-,O2·-and H2O2 were 56.94％,64.08％ and 31.69％ respectively.[Conclusion]Under the optimal culture conditions,EPS yield reached the maximum value of 6.16 mg/mL,which was 24.9％ higher than that before. Antioxidant activity results showed that EPS had strong antioxidant activity.

关键词

胞外多糖/培养条件优化/自由基清除/抗氧化活性

Key words

exopolysaccharides (EPS)/optimization of culture conditions/radical scavenging/antioxidant activ-ity

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出版年

2024

草原与草坪

中国草学会　甘肃农业大学

草原与草坪

CSTPCD

影响因子：0.686

ISSN：1009-5500

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