Optimization of culture conditions and antioxidant activity of exopolysaccharides produced by Pseudomonas simiae MHR6
[Objective]In this paper,the effects of culture conditions on the growth of Pseudomonas simiae MHR6 and the production of exopolysaccharides(EPS) were studied,and the antioxidant activity of P. simiae MHR6 was investigated,providing a reliable theoretical basis for the development and utilization of new agricultural biological agents.[Method]With the yield of EPS as the index,the EPS production conditions by MHR6 was opti-mized by the single factor test. Subsequently,the antioxidant activity was determined by OH-,O2·-and H2O2 scav-enging rate of EPS.[Result]The results showed that after optimization,the optimal culture conditions for MHR6 to produce EPS were as follows:culture time 5 d,temperature 28 ℃,initial pH value 8,inoculation amount 3％. When the mass concentration of EPS was 1.0 mg/mL,the scavenging rates of OH-,O2·-and H2O2 were 56.94％,64.08％ and 31.69％ respectively.[Conclusion]Under the optimal culture conditions,EPS yield reached the maximum value of 6.16 mg/mL,which was 24.9％ higher than that before. Antioxidant activity results showed that EPS had strong antioxidant activity.
exopolysaccharides (EPS)optimization of culture conditionsradical scavengingantioxidant activ-ity
万方数据
维普
