首页|2株耐盐碱根际促生细菌对盐碱胁迫下紫花苜蓿生长及生理的影响

2株耐盐碱根际促生细菌对盐碱胁迫下紫花苜蓿生长及生理的影响

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[目的]研究接种耐盐碱根际促生细菌嗜麦芽寡养单胞菌JT4、普城沙雷氏菌JG1对盐碱胁迫条件下紫花苜蓿生长和生理特性的影响,为根际促生细菌JT4、JG1在植物抗盐碱中的应用提供理论依据.[方法]采用室内盆栽试验,以紫花苜蓿为供试植物,以实验室前期筛选鉴定得到的耐盐碱及促生能力均较好的根际促生细菌JT4、JG1为供试菌株,设置盐碱胁迫及非盐碱胁迫处理,测定接种根际促生细菌JT4、JG1后紫花苜蓿幼苗生长和生理特性指标.[结果]与对照相比,盐碱胁迫下接种JT4、JG1紫花苜蓿幼苗的株高分别提高了31.64%和43.50%,根长分别提高了32.78%和7.05%,鲜重分别提高了100%和60%,抗坏血酸过氧化物酶活性分别提高了27.67%和200.58%,过氧化氢酶活性分别提高了33.33%和22.73%,可溶性蛋白含量分别提高了126.94%和118.88%,叶绿素a含量分别提高了11.12%和16.32%.[结论]耐盐碱根际促生细菌JT4、JG1能较好地缓解盐碱胁迫对紫花苜蓿造成的损伤.
Effects of two saline-tolerant rhizosphere growth-promoting bacteria on growth and physiology of Medicago sativa under saline-alkali stress
[Objective]In this experiment,the effects of inoculation of halotolerant rhizosphere growth-promoting bacteria,Stenotrophomonas maltophilia JT4 and Serratia plymuthica JG1,on plant growth and physiological charac-teristics of Medicago sativa under saline-alkali stress were studied,so as to provide theoretical basis for the applica-tion of rhizosphere growth-promoting bacteria JT4 and JG1 in saline-alkali resistance of plants.[Method]In indoor pot experiment,alfalfa was used as the test plant,and rhizosphere growth-promoting bacteria JT4 and JG1 with good saline-alkali tolerance and growth-promoting ability were used as the test strains. The effects of inoculation of rhizo-sphere growth-promoting bacteria JT4 and JG1 on the growth and physiological characteristics of alfalfa seedlings were studied under saline-alkali stress and non-saline stress.[Result]Compared with the control,the plant height,root length,fresh weight,ascorbic acid peroxidase activity and catalase activity of alfalfa seedlings inoculated with JT4 and JG1 increased by 31.64% and 43.50%,32.78% and 7.05%,100% and 60%,27.67% and 200.58%,and 33.33% and 22.73% respectively,soluble protein content increased by 126.94% and 118.88%,and chlorophyll a content increased by 11.12% and 16.32%,respectively.[Conclusion]Saline-alkali tolerant rhizosphere growth-pro-moting bacteria JT4 and JG1 can better alleviate the damage of alfalfa caused by saline-alkali stress.

rhizosphere growth-promoting bacteriaalfalfasaline-alkali stressantioxidant activity

陈露、马南楠、陈宁、曲善民、苗阳阳

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黑龙江八一农垦大学动物科技学院,黑龙江 大庆 163319

北大荒现代农业产业技术协同创新中心,黑龙江 大庆 163319

黑龙江省寒区饲料资源高效利用与营养调控重点实验室,黑龙江 大庆 163319

根际促生细菌 紫花苜蓿 盐碱胁迫 抗氧化活性

2024

草原与草坪
中国草学会 甘肃农业大学

草原与草坪

CSTPCD
影响因子:0.686
ISSN:1009-5500
年,卷(期):2024.44(4)