Effects of pink pigment from Erwinia persicina Cp2 on seed germination and seedling growth physiology of Medicago sativa
[Objective]This study investigates the effects of the pink pigment produced by Erwinia persicina Cp2 on seed germination and seedling growth physiology in alfalfa(Medicatgo sativa'Juneng 551').[Method]Dif-ferent concentrations of Cp2 pink pigment(0,0.094,0.188,0.282,0.376,0.470 mg/mL)were applied to alfalfa seeds,and various indicators related to germination,seedling growth and physiological characteristics were measured.[Result]The findings indicate that:1)Cp2 pink pigment did not significantly affect seed germination.2)There was a concentration-dependent effect on seedling growth;with the increase of Cp2 pink pigment concentration,symptoms of leaf yellowing and wilting became more pronounced.The root systems of alfalfa seedlings tended to be shorter and thicker,with significantly lower chlorophyll content compared to the control(CK).The root length and chlorophyll content decreased to their lowest levels,whilethe relative electrical conductivity of leaves peaked at the 0.470 mg/mL treatment.3)Compared to the control,Cp2 pink pigment significantly decreased the activities of superoxide dismutase and catalase in alfalfa seedlings.Treatments at concentrations of 0.188～0.470 mg/mL induced significant increases in superoxide anion,hydrogen peroxide,and malondialdehyde levels.4)Correlation and principal compo-nent analyse srevealed substantial differences in seedling growth due to different concentrations of Cp2 pink pigment,primarily reflected in the inhibition of root growth,chlorophyll degradation,and reduced antioxidant enzyme activities.[Conclusion]In summary,the pink pigment secreted by Erwinia persicina Cp2 significantly inhibits the growth and development of alfalfa seedlings.These results provides a theoretical foundation for understanding the pathogenic mechanism of Erwinia persicina Cp2 and for developing control strategies against bacterial disease in alfalfa.
万方数据
维普
