[Objective]To determine the optimal formulafor solid-state fermentation of Trichoderma afroharzia-num AD-G4.[Method]This study employed single factor test and Box-Behnken Design(BBD)response surface methodologyto optimize the solid fermentation medium and conditions for the T.afroharzianum AD-G4 strain.[Result]The findings indicated that the optimal medium composition consisted of a 1∶1 ratio of oat flour to wheat straw.The ideal culture conditions were determined to be an initial pH of 7.0,water content of 52%,spore concentra-tion of 5.40×106 spores/mL,a temperature of 28℃,and a culture durationof 8 days.[Conclusion]Under these cul-ture conditions,conidia yield of T.afroharzianum AD-G4 reached 1.91×1010 spores/g.The optimized culture condi-tions provide a foundation for the development of efficient T.afroharzianum biocontrol agent.
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