Molecular characterization and functional analysis of CYP3A2 gene in plateau zokor and plateau pika
[Objective]This study aimed toinvestigate the molecular structure and spatial conformation of the CYP3A25 gene in plateau zokor(Eospalax baileyi)and plateau pikas(Ochotona curzoniae).[Method]Nucleotide sequences of the CYP3A25 gene exons were detectedin both species and,and amino acid sequences and protein spa-tial conformations were analyzed in comparison with seven other rodent species.[Result]PCR successfully ampli-fiedthe CDS region of the 1 307 bp-long CYP3A25 genefor bothzokors and pikas,revealingdifferences in gene se-quence and protein spatial conformation.A phylogenetic tree was constructed,demonstrating that the plateau zokor and the blind mole(Nannospalax galili)were closely related,while the plateau pika was more comparable to humans(Homo sapiens).Analysis of CYP3A25 gene variation revealedthree amino acid mutations in plateau zokor and pla-teau pika,resulting in changes to protein spatial conformation compared to Mus.[Conclusion]This study identifies-significant sequence differences in the CYP3A25 gene among different species,impacting the spatial conformation of proteins.These findings suggestimportant variations in molecular characteristics and potential detoxification metabo-lism across species,providing insights into genetic differences in detoxification pathways.
万方数据
维普
