摘要
[目的]探究桃色欧文氏菌Cp2 脂多糖(Erwinia persicina Cp2 Lipopolysaccharides,Cp2-LPSe)对秀丽隐杆线虫(Caenorhabditis elegans)生长发育的影响.[方法]以野生型秀丽隐杆线虫N2为材料,用试剂盒法提取纯化Cp2-LPSe,用不同浓度Cp2-LPSe(0、0.157、0.314、0.471、0.628 与0.785 EU/mL)处理秀丽隐杆线虫,测定其咽泵次数、繁殖能力、体脂含量、寿命长短、运动速度、运动振幅、身体弯曲频率等指标.[结果]各Cp2-LPSe浓度的处理下,秀丽隐杆线虫的生长发育总体呈现先上升后下降的趋势,当浓度低于0.314 EU/mL Cp2-LPSe时,除体脂含量外各指标均显著高于对照(P<0.05),并在0.314 EU/mL达到最大值;浓度超过0.314 EU/mL时,各指标均显著低于对照(P<0.05).[结论]低于0.314 EU/mLCp2-LPSe处理秀丽隐杆线虫会提高其咽泵次数、运动速度、身体弯曲频率、运动振幅,在体脂含量不增高的前提下提高其繁殖能力、延长其寿命,促进其生长发育.本研究为Cp2-LPSe作为一种延缓机体衰老制剂的开发提供理论依据.
Abstract
[Objective]The study is aimed to investigate the effect of Cp2 Lipopolysaccharides(Cp2-LPSe)of Erwinia persicina on the growth and development of Caenorhabditis elegans.[Method]In this experiment,Cp2-LPSe was extracted and purified by kit method using wild-type Caenorhabditis elegans N2 as the material.Different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)were used to treat Caenorhabdi-tis elegans with different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)to deter-mine the number of throat pumps,reproductive capacity,body fat content,life span,movement speed,movement am-plitude,body bending frequency and other indicators.[Result]The experiment showed that the growth and develop-ment of Caenorhabditis elegans had a general trend of first rising and then decreasing under the treatment of each Cp2-LPSe concentration.When the concentration of Cp2-LPSe was 0.314 EU/mL,all indexes except body fat content reached a maximum and were significantly higher than those of the control(P<0.05),and when the concen-tration exceeded 0.314 EU/mL,all indexes were significantly lower than those of control(P<0.05).[Conclusion]0.314 EU/mL Cp2-LPSe treatment of Caenorhabditis elegans could increase the number of throat pumps,move-ment speed,body bending frequency and movement amplitude,improve its reproductive ability,prolong its lifespan,and promote its growth and development without increasing its body fat content.This study provides a theoretical ba-sis and practical basis for the development of Cp2-LPSe as a anti-aging agent.
维普
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