Effect of Cp2 lipopolysaccharides of Erwinia per-sicina on the growth and development of Caenorhabditis elegans
[Objective]The study is aimed to investigate the effect of Cp2 Lipopolysaccharides(Cp2-LPSe)of Erwinia persicina on the growth and development of Caenorhabditis elegans.[Method]In this experiment,Cp2-LPSe was extracted and purified by kit method using wild-type Caenorhabditis elegans N2 as the material.Different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)were used to treat Caenorhabdi-tis elegans with different concentrations of Cp2-LPSe(0,0.157,0.314,0.471,0.628 and 0.785 EU/mL)to deter-mine the number of throat pumps,reproductive capacity,body fat content,life span,movement speed,movement am-plitude,body bending frequency and other indicators.[Result]The experiment showed that the growth and develop-ment of Caenorhabditis elegans had a general trend of first rising and then decreasing under the treatment of each Cp2-LPSe concentration.When the concentration of Cp2-LPSe was 0.314 EU/mL,all indexes except body fat content reached a maximum and were significantly higher than those of the control(P<0.05),and when the concen-tration exceeded 0.314 EU/mL,all indexes were significantly lower than those of control(P<0.05).[Conclusion]0.314 EU/mL Cp2-LPSe treatment of Caenorhabditis elegans could increase the number of throat pumps,move-ment speed,body bending frequency and movement amplitude,improve its reproductive ability,prolong its lifespan,and promote its growth and development without increasing its body fat content.This study provides a theoretical ba-sis and practical basis for the development of Cp2-LPSe as a anti-aging agent.
Cp2 Lipopolysaccharides of Erwinia persicinaCaenorhabditis elegansgrowth and development
万方数据
维普
