Comprehensive Evaluation of Antioxidant Capacity of Iris lactea Accession Petals
[Objective]In order to explore the differences in antioxidant capacity of petals of various germplasm materials of Iris lactea Pall.var.chinensis(Fisch.)Koidz.,the accessions of I.lactea with strong antioxidant capacity were screened out,which would lay a foundation for the evaluation,development and utilization of Iris lactea acces-sions.Method In this paper,22 I.lactea accessions under different habitat conditions in 6 provinces of China were selected to determine anthocyanin,flavonoid,total phenol,proanthocyanidin,hydroxyl radical scavenging rate and to-tal antioxidant capacity of petals at full flowering stage,and the correlation between each index was analyzed.At the same time,the antioxidant capacity of each material was comprehensively evaluated by principal component analysis combined with membership function method.[Result](1)The results showed that there were some differences in the content of each component among 22 I.lactea accessions.Among them,the coefficient of variation of proanthocy-anidin content was the largest(64.41%),and the coefficient of variation of total antioxidant capacity content was the smallest(4.53%).The anthocyanin content of the 22 samples was 0.15～0.56 mg/g,the flavonoid content was 0.51～1.34 mg/g,the total phenol content was 1.19～1.90 mg/g,the proanthocyanidin content was 0～0.19 mg/g,and the hydroxyl radical scavenging rate ranged from 24.97%to 50.82%.The total antioxidant capacity was be-tween 1.98 µmol Trolox/g and 2.36 µmol Trolox/g.(2)The results of correlation analysis showed that there were different degrees of correlation among the six physiological and biochemical indexes.According to the results of Eu-clidean distance cluster analysis,the antioxidant capacity of 22 I.lactea accessions could be clustered into three catego-ries.,of which 8 had strong antioxidant capacity,including ML011,ML012,ML013,ML014,ML016,ML019,ML029 and ML035,9 had moderate antioxidant capacity and 5 had weak antioxidant capacity.(3)The results of comprehensive comparison of antioxidant capacity of I.lactea accessions by principal component analysis combined with membership function method were ML014>ML013>ML011>ML012>ML035>ML19>ML016>ML029>ML018>ML008>ML010>ML006>ML020>ML005>ML009>ML007>ML023>ML017>ML032>ML015>ML004>ML001,which were highly consistent with the results of cluster analysis.[Conclusion]The above research shows that ML011,ML012,ML013,ML014,ML016,ML019,ML029 and ML035 have strong antioxidant capacity in 22 I.lcateaaccessions,which provides scientific basis for the excavation of excellent Iris germplasm resources and the development and utilization of products.
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