[目的]探究植物根际促生菌对垂穗披碱草在盐渍化土壤中生长的影响.[方法]以课题组前期分离的4株植物根际促生菌(PGPR)为试验材料,设置不同盐浓度处理,采用沙培法种植垂穗披碱草(Elymus nutans),将4株植物根际促生菌[(TznFn7、MYnI4、MYP(m)Jn8和TznG2)]接种剂接种在种植垂穗披碱草的河沙中,测定不同菌株处理和不同盐浓度下垂穗披碱草生长指标和生理特性,研究垂穗披碱草在菌株接种剂处理下的耐盐性.[结果]在 150 mmol/L盐浓度下,与CK相比各接种处理的CAT 活性提高 2.08%～7.35%,接种菌株 TznFn7、TznG2 处理下与 CK 相比 POD 活性提高了27.24%、13.30%,各接种处理下SOD活性提高 5.18%～24.03%,与CK相比各接种处理的MDA含量降低25.87%～47.53%.[结论]在盐胁迫下,接种不同PGPR菌剂不仅促进垂穗披碱草生长,同时有效提高了植物CAT、POD和SOD抗氧化酶活性,降低了植物细胞MDA含量.本研究选用的PGPR菌株改善了盐胁迫下植物生长,减轻了植物细胞损伤,提高了植物耐盐性,为盐渍化土壤的利用提供了一定的理论和实践基础.
Effects of plant growth-promoting rhizobacteria on growth,physiological,and biochemical responses to salt-stressed Elymus nutans
[Objective]This study investigated the effects of plant growth-promoting rhizobacteria(PGPR)on the growth of Elymus nutanssalinized soil.[Method]Four plant PGPR strains isolated by our research group(TznFn7,MYnI4,MYP(m)Jn8 and TznG2)were tested under varying salt concentrations.E.nutanswas cultivated in a sand culture system,andthe growth indices and physiological characteristics were assessed under different strain treatments and saltlevels.The study focused on salt tolerance of E.nutans following PGPR innoculation.[Result]Results demonstrated that at a salt concentration of 150 mmol/L,catalase(CAT)activity increased by 2.08%to 7.35%,while peroxidase(POD)activity rose by 27.24%and 13.30%with TznFn7 and TznG2 inoculations,com-pared to control(CK).Superoxide dismutase(SOD)activity increased between 5.18%and 24.03%across all in-noculatedstrains,and malondialdehyde(MDA)content decreased from 25.87%to 47.53%compared to CK.Inocula-tion with different PGPR strains not only promoted the growth of E.nutans under salt stress but also enhanced the ac-tivities of CAT,POD and SOD,while reducing MDA content in plant cells.This alleviated the cellular damage and improved the plantsresilience to saline conditions.[Conclusion]The PGPR strains selected in this study significantly enhanced plant growth and salt tolerance,providing a theoretical and practical basis for the utilization of saline soils.
salt stress:plant growth-promoting rhizobacteria:Elymus nutans:physiologicresponse
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