摘要
[目的]明确沼液对生长期黄芪10种无机元素积累的影响,分析无机元素与有效成分的相关性.[方法]用不同浓度沼液分别对黄芪进行叶面喷施、土壤施肥和叶面喷施+土壤施肥,研究其对黄芪无机元素和有效成分含量的影响.[结果]沼液叶面喷施,以80%沼液处理的Fe、K、Ca、Mg、Na、Ni含量较高,显著高于其他处理(P<0.05).沼液土壤施肥,采收期数据显示:80%沼液土壤施肥处理的Fe、K、Ca、Na、Mn含量显著高于其他10个处理(P<0.05),Ni含量以CF处理值最高,显著高于其他处理(P<0.05).相关性分析表明,Fe含量与毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素含量,Na含量与芒柄花素含量呈显著正相关;Mg含量与芒柄花苷、毛蕊异黄酮含量,Na与黄芪皂苷Ⅲ含量均存在显著负相关.判别分析发现造成沼液与其他处理差异的主要元素有Ca、Na、Mn、Fe、Mg.[结论]适宜的沼液施用模式可促进黄芪根无机元素的累积,且与有效成分存在密切关系.
Abstract
[Objective]This study investigates the effect of biogas slurry application on the accumulation of ten inorganic elements in Astragalus membranaceus var.mongholicus during growth,and analyzes the correlation be-tween these inorganic elements and active ingredients.[Method]The impact of biogas slurry concentration on the ac-cumulation of inorganic elements and active compounds in Astragalus membranaceus var.mongholicus was examined using three application methods:foliar spraying,soil fertilization and a combination of both.[Result]Biogas slurry ap-plication significantly promoted the accumulation of inorganic elements in Astragalus membranaceus var.mongholi-cus.When biogas slurry was applied solely as foliar spray,the 80%concentration treatment notably increased the con-tents of potassium(K),calcium(Ca),magnesiusm(Mg),sodium(Na)and nickel(Ni),performing significantly bet-ter than the other treatments(P<0.05).In soil fertilization treatments,the harvest data revealed that 80%biogas slurry topdressing treatment resulted in significantly higher concentrations of zinc(Zn),iron(Fe),K,Ca,Na,and manganese(Mn)compared to the other 10 treatments(P<0.05).Conventional fertilization yielded the highest Ni content in Astragalus membranaceus var.mongholicus compared to other treatments(P<0.05).Correlation analy-sis indicated significant positive correlations between Fe and the contents of calycosin-7-O-β-D-glycosides,ononin,ca-lycosin,and formononetin.Na showed a significant positive correlation with formononetin,while Mg was negatively correlated with ononin and formononetin.Additionally,Na had a negative correlation with astragaloside III content.Discriminant analysis identified Ca,Na,Mn,Fe,and Mg as the primary elements responsible for the differences be-tween the biogas slurry and other treatments.[Conclusions]Optimal concentrations and application methods of bio-gas slurry can enhance the accumulation of inorganic elements in Astragalus membranaceus var.mongholicus,with the contents of active ingredients closely linked to these inorganic elements.
维普
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