Study on endogenous inhibitors and hormonal changes during low-temperature accumulation of Pterocephalus hookeri seeds
[Objective]The study was carried out in order to explore the internal relationship between seed physi-ological and biochemical changes of Pterocephalus hookeri and dormancy removal during the process of low tempera-ture stratification.[Method]The seed dormancy was relieved by low temperature stratification treatment(60 d).The germination rate,endogenous inhibitors and the activities of gibberellin(GA),indoleacetic acid(IAA)and ab-scisic acid(ABA)were observed at different stratification time.[Result]The results showed that during the low tem-perature stratification process,the germination rate of the seed was significantly increased at the late stage of stratifica-tion(45～60 d)(P<0.05).After stratification,GA content was significantly increased(P<0.05),and the ratio of germination promoters to inhibitors(GA/ABA,IAA/ABA,GA+IAA/ABA)also showed an increasing trend.There was a significant positive correlation between seed germination rate and GA(P<0.05).[Conclusion]It was found that there were endogenous inhibitors in the seeds of Pterocephalus hookeri.With the extension of the stratifica-tion time,the inhibition of the seed extract on the germination rate,root length and cotyl length of P.hookeri seeds first increased and then decreased.The increase of GA content was also the key factors for the release of dormancy of the seeds.
万方数据
维普
