Effect of rhizobia inoculation on vegetative organ biomass of alfalfa
[Objective]To explore the biomass changes and symbiotic effect differences in various organs of Al-falfa Seedlings under different rhizobial inoculation environments,[Method]Medicago sativa(WL289HQ,WL319HQ,WL363HQ,Longzhong,Qingshui,Gannong 9)were used as materials.Rhizobia strains G3L3,G3L4,G3L7,LL7,LP3,G9L4,WLP2,S12531 were inoculated as treatments.Samples without rhizobia inoculation were-usedas control.The differences among M.sativa cultivars after inoculation with rhizobia,the differences among rhizo-bial strains,and the interactive effect differences between cultivars and strains were comparatively analyzed.[Result]Among different alfalfa varieties,WL298HQ had the highest biomass of root,stem and leaf.Among different mycor-rhizal treatments,the root and leaf biomass of G9L4 was the highest,while the stem biomass of G3L7 was the high-est.Under the interactive effect of variety and strain,the WL298HQ alfalfa inoculated with strains G3L3,LL7,G9l4,and LP3 as well as the gannong 9 alfalfa inoculated with strains G3L3,G3L7 showed enhanced leaf,stem,and root biomass accumulation.Longzhong inoculated with strains G3L3,WLP2,LL7,and LP3 as well as the Qingshui in-oculated strain WLP2 in longum showed inhibitory effects on the accumulation of leaf,shoot,and root biomass.WL298HQ showed the strongest partition effect with strain LL7 on the biomass of stem and leaf,with the best match-ing effect.[Conclusion]The WL298HQ and Gannong 9 inoculated strain G3L3 showed positive effect on leaf,stem and root biomass accumulation.The inhibitory effect of WLP2,an inoculated strain with long neutralizing Medicago clear,on the accumulation of leaf,shoot and root biomass,and the partition effect of the symbiosis between M.sativa and rhizobia on biomass dry weight followed:stem>leaf>root.
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