Response of non-structural carbohydrates content to low phosphorus stress of sainfoin
[Objective]This study aimed to investigate the changes and responses of non-structural carbohy-drate and phosphorus content in low-phosphorus-tolerant and phosphorus-sensitive sainfoin under low phospho-rus stress.[Method]This experiment adopted a nutrient solution sand culture method in which materials 6,5,1,10 295 and Onobrychis viciaefolia'Gansu'were used as experimental materials.These materials were treated with con-stant normal phosphate(1.00 mmol/L KH2PO4,NP)and deficient phosphate stress(0.01 mmol/L KH2PO4,LP).Samples were collected at 0 d,12 d and 24 d,respectively,and the contents of soluble sugar,starch,reducing sugar,sucrose and phosphorus in stem and leaf,root were determined.[Result]Underlow-phosphorous stress,within the stems and leaves,soluble sugar,starch,and sucrose content initially reduced and then increased,while the reducing sugar content consistently decreased.In the roots,soluble sugar content and reducing sugar content showed a trend of decrease,starch content initially increased and then decreased,and sucrose contentinitially decreased and then in-creased.Phosphorus content decreased with increasing duration of stress.[Conclusion]Based on a comprehensive analysis using the membership function method,material 5 had a strong tolerance to low-phosphorus,and material 1 had a weak tolerance low-phosphorus.
sainfoinlow phosphorus stressnon-structural carbohydrate contentmembership function method
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