Study on callus inducement and plant regeneration in seeds of Aneurolepidium chinense (Trin) Kitag
The experiment studies on the technology of plant tissue culture in three kinds of Aneurolepidium chinense(Trin)Kitag seeds which are cultured in the basic culture medium MS within three levels concentrations of 2, 4-D(1, 2, 4 mg·L-1). During this experimentation, whose the inducement purpose is the best of all is the culture medium of MS supplied with 1 mg·L-1 2, 4-D. In roots culture, the result of using half-strength (1/2) MS is better. The culture results, that the seeds of Aneurolepidium chinense(Trin) Kitag are cultured in culture mediums from screening test, have much virtue, such as higher callus induction rate, better callus quality, higher seedling polarization rate, stronger seedling.