The DH population of 6 combinations had been constructed in cold rice region by the technology of antheral culture in hybridization combination F1 which was from 10 parents of different amylose content. Analyzed the hereditary variation distribution of amylose content in DH population, estimated hereditary capacity and rate over parent in DH population by the model and formul which were presented by Choo and Reibergs, and examined the number of gene pairs and the pattern of gene interaction which controlled these characters. The hereditary variation of amylose content was divided into two types in this paper. Type Ⅰ was the heredity of 5 crosses of amylase content which belonged to blending heredity which was controlled by major gene which had hereditary feature of quantitative character and many minor genes, there was no interaction of major genes. Type Ⅱ was the heredity of the amylase content of only one cross (Song9798/Longhua96-1530) which belonged to blending heredity controlled by many major genes that had hereditary feature of quantitative character and many minor genes; there was epistasis effect between major genes, which was over lapped effect. The hereditary capacity of amylose content was 0.90 in DH population, the rate over low parent was relatively low. The number of gene pairs was 4.6-18.1 pairs controlled by the amylose content, 7.5 on average. Therefore, the suitable DH population for culture quality breeding practice should be kept about 100 and inoculate the anther of F1 or F2 generation according to different situations for enhancing the selected intensity in H2 generation. It should not be selected too strictly in early generation in breeding for amylase content.