Screening of simple and fast method to observe leaf anatomical structure
The methods used in observing the morphological and anatomical structure of plant have some problemes, such as high cost, time-consuming, inconvenient to operate and so on. The article screened simple and quick observation methods to solve these issues. Food gum has plasticity, transparency, and gumminess. The research tried to use these features to get the cell traces of plant, and through the microscope indirect observed the anatomical structure. For teaching and scientific research to provide a simple and rapid new method to enrich the production technology of botany. The results showed that gelatin was suitable for getting the epidermal cell and transaction cell traces, and free hand section method was suitable for preparation the petiole transaction anatomical structure. Using 95% ethanol to decolour could get the best effect, but need to immersion in the first concentration in the gradient of 20%, 50%, and 70% of the ethanol solution in 2 h, which made leaves gradually dehydrated, and prevented leaves dewatering and deforming.
leafmorphological and anatomical structureobservation methodscreening
NETL
NSTL
万方数据
维普
